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凡纳滨对虾敲降β-catenin后感染白斑综合症病毒的转录组分析

Transcriptome analysis of Penaeus vannamei after knocking-down of β-catenin post white spot syndrome virus infection

  • 摘要: 白斑综合症病毒(white spot syndrome virus, WSSV)是对虾养殖过程中最具代表性的病原体之一,给对虾养殖产业造成了严重的经济损失。Wnt/β-catenin信号通路是高度保守的信号通路,越来越多的研究表明,该通路在宿主免疫过程中发挥重要作用。本研究以凡纳滨对虾(Penaeus vannamei) Wnt/β-catenin信号通路的核心分子Lvβ-catenin为研究对象,采用RNA干扰技术敲低其转录水平,结合转录组学分析WSSV感染过程中Lvβ-catenin介导调控的响应分子。通过转录组差异表达基因比较分析与qRT-PCR验证,发现105个基因与WSSV感染过程中Lvβ-catenin介导的调控相关,其中免疫相关分子cGMP依赖蛋白激酶、血蓝蛋白F链、岩藻糖基转移酶8在受到WSSV刺激后转录水平下调,而在敲降Lvβ-catenin之后,血蓝蛋白F链、岩藻糖基转移酶8的转录水平会有所上调。利用GO、KEGG等数据库对转录组数据进行分析,结果显示差异基因的功能注释到代谢过程的基因数目最多,并且富集的通路主要集中于新陈代谢,表明WSSV侵染过程中β-catenin主要调控宿主的代谢过程,并可能通过调控宿主代谢过程来参与宿主的抗病毒过程。此外,分析还发现β-catenin可以调控下游抗菌肽的表达,从而帮助宿主抵御病毒侵染。综上所述,糖/氨基酸代谢和抗菌肽调控可能与宿主-病毒相互作用密切相关。本研究初步揭示了凡纳滨对虾响应WSSV感染时Lvβ-catenin介导的分子调控特征,将有助于深入认识Wnt/β-catenin信号通路在宿主-病毒相互作用过程中的功能机制。

     

    Abstract: White spot syndrome virus (WSSV) is one of the most representative pathogens in prawn aquaculture, which has caused serious economic losses. The Wnt/β-catenin signaling pathway is a highly conserved signaling pathway in vivo, and more and more studies show that this pathway plays an important role in host immunity. This article takes Lvβ-catenin, the core molecule of the Wnt/β-catenin signaling pathway in Penaeus vannamei, as the research object. RNA interference technology was used to knocking down its transcription, and the responsive molecules mediated by Lvβ-catenin during WSSV infection were screened by transcriptome analysis. Combination of transcriptome analysis and qRT-PCR verification, 105 genes were found to be related to regulation mediated by Lvβ-catenin during WSSV infection, including immune-related genes cGMP-dependent protein kinase, hemocyanin F chain-like, and alpha-(1,6)-fucosyltransferase-like. The transcription level of hemocyanin F chain-like, and alpha-(1,6)-fucosyltransferase-like is down-regulated after being stimulated by WSSV, while after knocking down Lvβ-catenin, the transcription levels of hemocyanin F chain-like and alpha-(1,6)-fucosyltransferase-like are up-regulated. Transcriptome data were analyzed using databases such as GO and KEGG. Results show that the functional annotation of differential genes was the largest number of genes related to metabolic processes, and the enriched pathways were mainly concentrated in metabolism, indicating that β-catenin mainly regulated the host’s metabolic process during WSSV infection, and might participate in the host’s antiviral process by regulating the host’s metabolic process. In addition, the analysis also found that β-catenin can regulate the expression of downstream antimicrobial peptides, thereby helping the host resist viral infection. Taken together, sugar/amino acid metabolism and antimicrobial peptide regulation may be closely related to host-virus interactions. This study preliminarily reveals the molecular regulatory characteristics mediated by Lvβ-catenin in Penaeus vannamei in response to WSSV infection, which will help to further understand the functional mechanism of the Wnt/β-catenin signaling pathway in the host-virus interaction process.

     

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