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YANG Zhongming, WU Wenhao, SHI Hong, HUANG Zhihao, RUAN Lingwei. Transcriptome analysis of Penaeus vannamei after knocking-down of β-catenin post white spot syndrome virus infection[J]. Journal of Applied Oceanography, 2025, 44(3): 444-455. DOI: 10.3969/J.ISSN.2095-4972.20231107001
Citation: YANG Zhongming, WU Wenhao, SHI Hong, HUANG Zhihao, RUAN Lingwei. Transcriptome analysis of Penaeus vannamei after knocking-down of β-catenin post white spot syndrome virus infection[J]. Journal of Applied Oceanography, 2025, 44(3): 444-455. DOI: 10.3969/J.ISSN.2095-4972.20231107001

Transcriptome analysis of Penaeus vannamei after knocking-down of β-catenin post white spot syndrome virus infection

  • White spot syndrome virus (WSSV) is one of the most representative pathogens in prawn aquaculture, which has caused serious economic losses. The Wnt/β-catenin signaling pathway is a highly conserved signaling pathway in vivo, and more and more studies show that this pathway plays an important role in host immunity. This article takes Lvβ-catenin, the core molecule of the Wnt/β-catenin signaling pathway in Penaeus vannamei, as the research object. RNA interference technology was used to knocking down its transcription, and the responsive molecules mediated by Lvβ-catenin during WSSV infection were screened by transcriptome analysis. Combination of transcriptome analysis and qRT-PCR verification, 105 genes were found to be related to regulation mediated by Lvβ-catenin during WSSV infection, including immune-related genes cGMP-dependent protein kinase, hemocyanin F chain-like, and alpha-(1,6)-fucosyltransferase-like. The transcription level of hemocyanin F chain-like, and alpha-(1,6)-fucosyltransferase-like is down-regulated after being stimulated by WSSV, while after knocking down Lvβ-catenin, the transcription levels of hemocyanin F chain-like and alpha-(1,6)-fucosyltransferase-like are up-regulated. Transcriptome data were analyzed using databases such as GO and KEGG. Results show that the functional annotation of differential genes was the largest number of genes related to metabolic processes, and the enriched pathways were mainly concentrated in metabolism, indicating that β-catenin mainly regulated the host’s metabolic process during WSSV infection, and might participate in the host’s antiviral process by regulating the host’s metabolic process. In addition, the analysis also found that β-catenin can regulate the expression of downstream antimicrobial peptides, thereby helping the host resist viral infection. Taken together, sugar/amino acid metabolism and antimicrobial peptide regulation may be closely related to host-virus interactions. This study preliminarily reveals the molecular regulatory characteristics mediated by Lvβ-catenin in Penaeus vannamei in response to WSSV infection, which will help to further understand the functional mechanism of the Wnt/β-catenin signaling pathway in the host-virus interaction process.
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