Advanced Search
SUN Yan, XIAO Peiyi, LIU Yuping, YANG Tianyan. Population genetic diversity analysis of Sillago sinica based on mitochondrial DNA COⅠgene and D-loop sequence[J]. Journal of Applied Oceanography, 2025, 44(3): 433-443. DOI: 10.3969/J.ISSN.2095-4972.20240521001
Citation: SUN Yan, XIAO Peiyi, LIU Yuping, YANG Tianyan. Population genetic diversity analysis of Sillago sinica based on mitochondrial DNA COⅠgene and D-loop sequence[J]. Journal of Applied Oceanography, 2025, 44(3): 433-443. DOI: 10.3969/J.ISSN.2095-4972.20240521001

Population genetic diversity analysis of Sillago sinica based on mitochondrial DNA COⅠgene and D-loop sequence

  • Sillaginidae is an important marine economic fish in China. Sillago sinica is a newly recorded species in this family, identifying its genetic characteristics and phylogenic pattern can provide references for rational fishery resource management. The mitochondrial COⅠgene and D-loop region were used as molecular markers to explore genetic diversity and genetic structure of four S. sinica populations (Qingdao, Rushan, Shengsi and Wenzhou) in coastal waters of China. The lengths of 614 bp (COⅠ) and 458 bp (D-loop) fragments were obtained after the polymerase chain reaction (PCR) and sequence alignment. A total of 11 and 13 polymorphic loci were detected based on COⅠgene and D-loop region, defining 7 and 9 haplotypes, respectively. Also, the shared haplotypes were identified among different populations. The average haplotype diversity (Hd) and nucleotide diversity (π) of D-loop region were 0.452 and 0.00260, which were slightly higher than those of COⅠgene (0.142 and 0.00044), indicating relatively low levels of genetic diversity. The genetic distances of different populations ranged from 0.00013 to 0.00062 (COⅠ) and from 0.00177 to 0.00350 (D-loop), respectively. The genetic differentiation index (Fst) and analysis of molecular variance (AMOVA) suggested that the majority of genetic variation resided within the populations. The neighbor-joining (NJ) tree showed geographical distribution patterns from the southern and northern sea areas. Qingdao and Rushan populations distributing in the Yellow Sea clustered together, while Shengsi and Wenzhou populations belonging to the East China Sea formed an independent clade. The analysis of population demographic history implied that S. sinica from the northern group might have experienced a population expansion event. However, the number and scale of S. sinica from the southern group were relatively stable with no obvious fluctuations. In summary, low genetic diversity together with significant genetic differences between divergent sea areas indicated that different fishery units of S. sinica should be managed separately in China.
  • loading

Catalog

    Turn off MathJax
    Article Contents

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return